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Description
Dopamine and serotonin are main neurotransmitters that maintain excellent mental wellbeing and regulate physiological functions in human tissues. Oxidation of both neurotransmitters is of great importance in understanding their mechanisms of actions in the human body. This scientific study reveals the most probable oxidation mechanisms of these neurotransmitters in simulated physiological conditions (phosphate buffer with pH = 7.4) using voltammetric techniques (cyclic and square-wave voltammetry).
Results were obtained using several commercial unmodified electrodes, glassy carbon electrode (GC), platinum electrode (Pt), basal plane pyrolytic graphite electrode (BPPG) and gold electrode (Au) and compared with the literature1,2. Different electrodes gave distinctive voltammetric signals, with slightly better results on both carbon electrodes. Using both techniques and scan rate (frequency in SWV) analysis, both oxidation mechanisms were discussed and explained in terms of the recently proposed ECE mechanism (dopamine)2 and ECEC mechanism (serotonin)3. Furthermore, fitting the experiments with theoretical simulations, we were able to find several values for key parameters of the proposed mechanisms.
Keywords: dopamine, serotonin, cyclic voltammetry, square wave voltammetry, electrodes.
References:
1. Sharma S et al. A review on electrochemical detection of serotonin based on surface modified electrodes, Biosens Bioelectron. 2018, 107, 76-93. DOI: 10.1016/j.bios.2018.02.013.
2. Bacil RP et al. Dopamine oxidation at gold electrodes: mechanism and kinetics near neutral pH, Phys Chem Chem Phys. 2020, 22(2), 607-614. DOI: 10.1039/c9cp05527d.
3. Martin C. Henstridge et al. Generator/Collector Experiments with a Single Electrode: Introduction and Application to Exploring the Oxidation Mechanism of Serotonin, The Journal of Physical Chemistry C. 2009, 113 (32), 14285-14289. DOI: 10.1021/jp904083e.
