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Description
Malathion is one of the most commonly used organophosphorus insecticides both in our country and world-wide. Its use is also approved in the countries of the European Union1. It is an active substance of the pesticide formulation Etiol techni, which is in the form of an emulsion concentrate (EC).
The development of new analytical methods for the determination of active substances in plant protection products is of great importance and need for the control of their quality.
This study presents a simple, precise and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of malathion in the pesticide formulation Etiol techni. The analysis was performed on a C-8 stationary phase using isocratic elution with mobile phase consisted of acetonitrile and water, flow rate of 1 mL/min, constant column temperature at 25 °C and ultraviolet-diode array detection (UV-DAD) at 220 nm. The specificity, selectivity, linearity, precision, accuracy, limit of detection (LOD) and quantification (LOQ) were tested for the method validation according to the CIPAC (Collaborative International Pesticides Analytical Council) guidelines. The calculated values for the recovery were ranged from 100.68 to 102.00 %, and the relative standard deviation (RSD) ranged from 0.15 to 1.36 %. The obtained results indicated that the proposed method can be used for routine analysis of the active ingredient malathion in the pesticide formulation Etiol techni following the CIPAC rules.
