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Description
The GC, GC-MS, and NMR techniques were utilized to examine the phytochemical composition of Microbiota decussata. A comparative analysis was performed with a previously published study conducted by Raldugin et al. in 1981.1 In contrast to the findings of the Russian group, our sample did not contain hedycaryol, which was identified as the major alcohol component. Instead, thujopsan-2α-ol dominated most of the sesquiterpenol-containing chromatographic fractions, comprising approximately one-third of the total extract mass. Furthermore, whereas Raldugin and colleagues could not detect any diterpenoids, we isolated totarol, constituting 10% of the extract, along with minor quantities of ferruginol. The most polar compound, microbiotol, accounting for 5% of the extract, was thoroughly examined using NMR and chiral GC to determine its relative and absolute configurations.
This work was supported by the Ministry of Science, Technological Development and Innovations of the Republic of Serbia (Contract Number 451-03-47/2023-01/ 200124).
Keywords: Microbiota decussata, thujopsan-2α-ol, totarol, NMR, GC-MS
References
1. Raldugin, V.A., Storozhenko, V.G., Rezvukhin, A.I., Pentegova, V.A., Gorovoi, P.G., Baranov, V.I. Terpenoids from plants of the family Cupressaceae. I. Sesquiterpene alcohols from the needles of Microbiota decussata. Chem. Nat. Compd. 1981. 17, 124–129. DOI: 10.1007/BF00634727
