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Description
A UV spectrophotometric method was validated to determine ciprofloxacin (CIP) in Citeral® eye/ear drops. A 0.1 mol/L HCl solution was used as a solvent for preparing standards and samples. The spectra of the samples were recorded in the range of 240–400 nm. The method demonstrated linearity in the range of 0.1–20 μg/mL, with a correlation coefficient exceeding 0.99999, obtained from absorption measurements at three wavelengths (277, 316, and 330 nm). The LOD and LOQ values at 277 nm were 0.10 μg/mL and 0.31 μg/mL, respectively, while at both 316 nm and 330 nm, the LOD and LOQ were 0.12 μg/mL and 0.36 μg/mL, respectively. The accuracy and precision were confirmed using a standard addition method conducted in triplicate at three concentration levels. Consequently, the mean recovery values ranged from 98.40-102.03% at 277 nm, 97.97-102.02% at 316 nm, and 97.76-102.35% at 330 nm, while the repeatability and reproducibility (expressed as RSD%) were below the maximum acceptable set by Horwitz criterion1 (2.39%). Using classical spectroscopy, the best repeatability was observed at 277 nm at a concentration of 6 μg/mL (0.151%) and the best reproducibility was observed at 9 μg/mL (0.630%). The CIP concentration in the formulation, with expanded measurement uncertainty (MU), was estimated at 3.00 ± 0.07 mg/mL. Using the derivative signal 1D267, the best repeatability and reproducibility were measured at 6 μg/mL (0.171% and 0.242%, respectively), estimating the CIP concentration with MU at 3.06 ± 0.05 mg/mL. Besides its simplicity, speed, accuracy, and precision, according to the Analytical GREEnness calculator2, and with an obtained coefficient of 0.82, this method is environmentally friendly and suitable for routine analysis of CIP in eye/ear drop formulations.
