2nd Conference on Green Engineering, Sustainable Materials, and Technologies for Circular Economy (GREEN CIRC '25)

DETERMINATION OF THE FULLY OXIDIZED OIL CONTENT USING FTIR SPECTROSCOPY AND MULTIVARIATE CALIBRATION

Not scheduled

20m

Amphitheatre (Faculty of Technology and Metallurgy, Ss Cyril and Methodius University in Skopje)

Oral presentation

Speaker

Fatos Rexhepi (University "Isa Boletini" Mitrovica Kosovo)

Description

DETERMINATION OF THE FULLY OXIDIZED OIL CONTENT USING FTIR SPECTROSCOPY AND MULTIVARIATE CALIBRATION

Fatos Rexhepi1,, Artan Gashi2
1 Faculty of Food Technology, University “Isa Boletini” Mitrovica Str. Ukshin Kovaqica N.N. 40000, Mitrovica, Republic of Kosovo
2 Faculty of Biotechnical Sciences, St. Kliment Ohridski University, Partizanska bb 7000, Bitola, Republic of North Macedonia
e-mail: fatos.rexhepi@umib.net

Plant oil during thermal treatment especially at the food industrial applications are sensitive to oxidation and their oxidative level is important point for the fried food quality. The existence of highly unsaturated fatty acids in edible oils leads to oxidative deterioration. In this research, the Fourier transform infrared technique was used to monitor and quantitavely determine the oxidation process of sunflower oil mixed with rapeseed oil usually applied in the frying of the potato chips. FTIR–ATR in combination with a chemometrics method, oxidative level quantively was rapidly determined using fully oxidized oil as a standard sample obtained by oxitest. Through preparation of the oil mixture in different concentration with fully oxidized oil was found that the IR characteristic peak range crucial for the oxidative level was 1550-1880 cm–1. The quantitative prediction model by PLSR regression models and with some pre-treatments such as Sawitzky-Golay smoothing were established to quantively determine the level of oxidized oil in edible oil using normal, 1st derivative and 2nd derivative spectra. PLSR model for 2nd derivative spectra showed best prediction as compared to PCR with coefficient of determination (R2) of 0.978 for calibration and 0.95 for validation.
The developed method is not expensive, does not require any sample preparation, is considered a green method of analysis, is very fast and highly precise. experimentally verified and calculated the lowest concentration of oxidized oil in unknown sample (LOD) of 7.33 % (v/v) and LOQ of 18.33 % (v/v), were determined.
Keywords: (Accelerated oxidation, FTIR, PLSR, fully oxidized, sunflower oil)