Speaker
Description
The work focuses on the development of biorefining technology from agricultural waste to produce simple sugars, organic compounds and second-generation biofuels. The necessity of such investigations lies in the constantly increasing expectations for replacement of fuels and chemical compounds originating from crude oil as well as to avoid utilization of agricultural crops to obtain food.
Current studies focus their main attention on the decomposition of alkali lignin using Bacillius sp. Experiments were carried out in a bench scale using flasks with a suspension volume of 100 cm3, placed in a thermostated shaker at the constant temperature of 30oC. The minimal medium was used here in order to provide essential nutrients for the bacteria growth, and to ensure fast lignin decomposition. Experiments were carried out in the triplicate tests. The photometric method was utilized to indicate presence of polyphenols, and to determine their concentration in the post-culture mixture. The duration of experiments for alkali lignin decomposition was up to 15 days. The amount of polyphenols obtained in each of the conducted trial was calculated per 1 g of dry mass.
The results of the presented studies will facilitate the selection of some relevant parameters for cultivation in the laboratory-scale bioreactors, bioprocess optimalisation and scale up.
| Scientific Sections | Green Chemistry |
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